Above: Tripping the light fantastic: the new Zeiss Lightsheet Z.1 fluorescence microscope.
A state-of-the-art, Zeiss Lightsheet Z.1 system has recently been installed within the Bioimaging Research Hub (BIOSI 2; E/0.03). The single plane illumination microscope (SPIM) , purchased through generous funding by Cardiff University’s Research Infrastructure Fund (Lead applicant: Dr Pete Watson), allows fast and gentle multi-channel 3D/4D fluorescence imaging at the sub-cellular level. The system offers the potential of whole organ imaging of fixed cleared samples and can provide an unprecedented insight into developmental processes occurring in live model organisms such as Arabadopsis thaliana (Thale Cress), Danio rerio (Zebrafish) and Drosophila melanogaster (Fruit fly), and in vitro organoid at a high spatio-temporal resolution. Further information about this system is available via the Bioimaging Hub equipment database.
Webinars:
- Zeiss Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Microscopy
- Zeiss Microscopy How to: Mount cleared samples in Lightsheet Z.1
Further reading:
- Zeiss website: Zeiss Lightsheet Z.1
- Zeiss website: Sample Preparation for Light Sheet Microscopy