Light Sheet Microscopy

What is light sheet microscopy and how can it help me in my research?

Light sheet microscopy – also referred to as Single Plane Illumination Microscopy (SPIM) – is a fluorescent light microscopical imaging technique whereby the specimen is illuminated only in a single plane at a time and detected from the perpendicular direction. Images are built up on a  layer by layer basis  allowing reconstruction of three-dimensional structures from the obtained images, similar to confocal microscopy. Unlike confocal, the technique does not require a spatial pinhole to eliminate out of focus light.

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