Above: the new Olympus VS200 high throughput slide scanning system in ECSCRI
A new, high throughput slide scanning system has recently been installed in the European Cancer Stem Cell Research Institute (ECSCRI) and is available for use as a spoke of the Bioimaging Research Hub. The equipment allows automated high-throughput scanning of histological samples via a range of image modalities, including epifluorescence. Further details of the system are available through the Hub’s equipment database. All enquiries for this system should be directed towards Mr Mark Bishop.
The eagle-eyed amongst you may have noticed that the main corridor within the Bioimaging Research Hub is looking a little nattier these days. We thought it required some brightening up and so have started to adorn its walls with some nice, new A0-sized foamex prints of microscopical images that we’ve generated in-house on the Hub’s microscope systems. We’ve tried to select images that showcase the beauty of the unseen microscopic world which reflect the art in science, or ‘SciArt’ as it is now commonly known. We hope the images stimulate interest and highlight the state-of-the-art research and facilities within Cardiff School of Biosciences. No prizes for spotting the artistic influences on some of our ‘works’ : )
Above: Some of the class microscopes in various states of dismantlement.
It goes without saying, to get the very best out of a microscope you need to know how to optimise and maintain its performance. That said, you’d be surprised just how many microscopists don’t know how to properly set up and maintain their microscopes.
Recently, we run our first microscope maintenance course as part of Cardiff University’s Continuous Professional Development (CPD) programme. We can’t tell you who it was for; but suffice to say, they use microscopes a lot in their work. The two day course covered the basics of light microscopy and the procedures necessary to keep a microscope squeaky- clean and correctly aligned. The practical element of the course saw delegates clean, rebuild and align both upright widefield and stereo-zoom microscopes.
Pleasingly, the course was well-received with very good to excellent feedback. Thanks to all who participated on the two busy but enjoyable days. Thanks must alsogo to our undergrad students for soiling and mis-aligning the microscopes ahead of the course – they did a far better job than we ever could ; )
Above: The new Ultimaker ‘Extended’ 3D printer building a stage insert for a novel microscope system.
The Bioimaging Research Hub has recently purchased an Ultimaker 3 ‘Extended’ professional 3D printer. The printer will allow users to 3D print bespoke pieces of scientific equipment or generate scale models of microscopic samples for use in their research as well as for teaching, outreach and engagement activities (examples can be found here andhere).
The 3D printer utilises FDM (Fused Deposition Modelling) printing technology and has a range of advanced features allowing the fabrication of professional quality, high resolution 3D prints. The printer can print in two different colours or a single colour in addition to a dissolvable PVA support scaffold, thus allowing complex overhanging structures to be printed at high fidelity whilst significantly reducing finishing time.
The printer has a large build volume (215 x 215 x 300mm), supports a range of materials (nylon, PLA, ABS, CPE and PVA) and has a print resolution of 20-200 microns. The printer is wi-fi enabled with an internal webcam so that users can remotely monitor the progress of their 3D prints.
Above: ‘Cutting-edge’ equipment: the Zeiss PALM MicroBeam laser micro-dissector located at the European Cancer Stem Cell Research Institute (ECSCRI)
A Zeiss PALM MicroBeam laser micro-dissector system is now available as a spoke of the Bioimaging Research Hub. The equipment, which is located at the European Cancer Stem Cell Research Institute (ECSCRI), allows isolation of DNA, RNA and protein from laser micro-dissected samples from both histological sections (paraffin wax or cryo) and live cells. Further details of the system are available through our equipment database. All enquiries for this system should be directed towards Mr Mark Bishop.
The old electron microscopy darkroom (BIOSI 2; E/0.05) within the Bioimaging Research Hub has recently been refurbished as a live cell imaging suite via generous support from BIOSI. It now houses a spinning disc confocal system for fast, live cell imaging applications. The system is based around an Olympus IX71 inverted microscope, kindly provided by Dr Pete Watson, which has been upgraded, via ISSF funding, with a Crest Optics X-Light V2 confocal head, a Cairn Research tri-line laser bank (405nm, 488nm, 561nm) and a Hamamatsu ORCA Flash 4 sCMOS digital camera with M-View Gemini image splitter. The system is fully integrated via Molecular Devices MetaMorph software and boasts a 40″ 4k display. The system will expand the Hub’s imaging toolbox, enabling high speed, multi-position, multi-colour 3D/4D image acquisition. Support systems for live cell imaging (i.e. gas and incubation) are also available within the facility. Further details of this system are available through our equipment database.
Above: surface-rendered confocal reconstructions of pollen samples (left) and their corresponding 3D printed models (right).
Isn’t the World Wide Web a wonderful thing? Not so long ago I wrote a short blog explaining how we had developed methodology to convert volume datasets from the confocal microscope into 3D printed models – perfect solid scale replicas of samples the size of a pollen grain etc. Well, shortly afterwards I received an email from someone who had not only read the blog but, serendipitously, wanted to do this very thing! What is more, she was located not a million miles away: in fact, little more than 400 yards down the road from us, working as a researcher within Cardiff University’s School of History, Archeology & Religion. Please excuse the pun, but it really is a small world!
Rhiannon Philp is an archaeologist – or palynologist to be precise – someone who studies ancient pollen grains and spores found at archaeological sites. Pollen extracted from archeological digs can be used for radiocarbon dating and for studying past climates and environments by identifying plants growing at the time. Rhiannon is using this information to develop an understanding of prehistoric sea level changes in South Wales as part of the Changing Tides Project.
Rhiannon asked if we could generate a reference collection of 3D pollen prints that could be used for teaching and outreach activities as part of a new Archaeology engagement project called Footprints In Time. Indeed, some of her pollen samples were from sites containing both human and animal footprints made over 5000 years ago!
You can see some of our results above: on the left are the surface-rendered confocal volume reconstructions and, on the right, their corresponding 3D printed facsimiles – courtesy of the BIOSI 3D printing facility.
If you’re at the National Eisteddfod in Abergavenny this week (29th July – 6th August), then please pop by to see Rhiannon’s stall within the Cardiff University tent – all of the models will be on display there, together with a lot more. Any further interest, then please get in touch.
Imagine being able to generate a highly accurate, solid scale replica of the sample that you are visualising down the microscope; a perfectly-rendered pollen grain, or blood cell, or microscopic organism, but big enough to hold and examine in your hand. It would allow much better 3D conceptualisation of the sample, particularly for blind or visually-impaired individuals, and would have enormous utility in teaching and in engagement activities, and what researcher wouldn’t want a tangible, physical embodiment of their research to help explain their work (and impress their colleagues) at scientific meetings? Sounds like the stuff of science fiction doesn’t it? Well, not any more. Thanks to 3D printing technology (and the help of Dr Simon Scofield‘s lab) we have started taking volume datasets from the confocal microscope out of the virtual world and making them a reality. If you would be interested in generating a highly accurate scale model of your favourite biological sample (or would simply like to handle a giant pollen grain!) then please feel free to get in touch.
The Bioimaging facility plays a significant role in public engagement and student recruitment activities within the School of Biosciences. This summer (June 19th, 2015) the Bioimaging Facility hosted large groups of students from St David’s Catholic sixth form college as part of the Universities Science, Technology, Engineering & Mathematics (STEM) annual conference. The event, organised by Dr Fiona Wyllie, was well-attended (with over 400 students) who engaged fully in the interactive exhibitions and demonstrations, which included confocal microscopy, macro-imaging and slide scanning microscopy. As part of the slide scanning demonstration, students were able to trial a virtual histology slide box – an online digital repository of over 400 high resolution histological images – that has been developed in-house by the Bioimaging Facility for School teaching purposes.
Not so long ago we received a strange request from Dr Sarah Perkins (BIOSI): could we accommodate a BBC camera crew within the Bioimaging Facility to film an episode of the Children’s CBBC television programme, Naomi’s Nightmares of Nature? The nightmare in question, was the eyelash mite, Demodex, a commensal ectoparasite that lives within the hair follicles (Demodex follicularum) and sebaceous glands (Demodex brevis) of the face, feeding off sebum and other organic detritus. Anyway, prior to filming, we spent an anxious morning attempting to isolate live Demodex from ‘volunteers’ faces by various means, including via sellotape, with little success Fortunately, when Naomi and her production team arrived, we struck gold! A few eyelashes extracted from their cameraman, Steve, revealed a bumper load of parasites and, using DIC optics, we were able to generate some nice microscopic footage of a family of mites tucking into their evening meal!