Publications | Bioimaging Hub News

The following are a selection of known publications involving research carried out within the Bioimaging Research Hub. If you use our facility please properly credit the support you have received and make us aware of any publications that arise through your work so that we can keep these pages up to date. Your continued support is essential to our success as a core facility.

The Bioimaging Hub should be cited in your manuscripts as “Cardiff University Bioimaging Hub Core Facility, RRID:SCR_022556″

Klipa, O., El Gammal, M., Hamaratoglu, F. (2023) Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation. J Cell Sci 136 (13): jcs259935. DOI: 10.1242/jcs.259935.

Board-Davies, E.L., Rhys-Williams, W., Hynes, D., Love, W.G., Williams, D.W. (2023) Antimicrobial effects of XF drugs against Candida albicans and its biofilms. Frontiers in Fungal Biology 4: 1225647. DOI: 10.3389/ffunb.2023.1225647.

Nassar, R., Nassar, M., Senok, A., Williams, D. (2023) Phytic Acid Demonstrates Rapid Antibiofilm Activity and Inhibits Biofilm Formation When Used as a Surface Conditioning Agent. Microbiol Spectrum 11(3):e0026723. DOI: 10.1128/spectrum.00267-23

Slesiona, N., Payne, L., Pope, I., Borri, P., Langbein, W., Watson, P. (2023) Correlative Extinction and Single Fluorophore Bleaching Microscopy for Ligand Quantification on Gold Nanoparticles. Adv. Mater. Interfaces 2023: 2300568 DOI: 0.1002/admi.202300568.

Webberley, T.S., Bevan, R.J., Kerry-Smith, J., Dally, J., Michael, D.R., Thomas, S., Rees, M., Morgan, J.E., Marchesi, J.R., Good, M.A., Plummer, S. F., Wang, D., Hughes, T.R. (2023) Assessment of Lab4P probiotic effects on cognition in 3xTg-AD Alzheimer’s disease model mice and the SH-SY5Y neuronal cell line. Int. J. Mol. Sci. 24, 4683. DOI: 10.3390/ijms24054683.

Hayes, A.J., Farrugia, B.L., Biose, I.J., Bix, G.J., Melrose, J. (2022) Perlecan, a multi-functional, cell-instructive, matrix-stabilizing proteoglycan with roles in tissue development has relevance to connective tissue repair and regeneration. Front Cell Dev Biol. 2022, 10: 856261. DOI: 10.3389/fcell.2022.856261.

Hayes, A.J., Whitelock, J., Melrose, J. (2022) Regulation of FGF-2, FGF-18, and transcription factor activity by perlecan in the maturational development of transitional rudiment and growth plate cartilages and in the maintenance of permanent cartilage homeostasis. J. Mol. Sci. 2022, 23(4): 1934. DOI: 10.3390/ijms23041934.

Smith, M.M., Hayes, A.J., Melrose, J. (2022) Pentosan polysulphate, a semisynthetic heparinoid disease-modifying osteoarthritic drug with roles in intervertebral disc repair biology emulating the stem cell instructive and tissue reparative properties of heparan sulfate. Stem Cells and Development. 31: 15-16. DOI: 10.3390/ijms23041934.

Best, H.L., Williamson, L.J., Lipka-Lloyd, M., Waller-Evans, H., Lloyd-Evans, E., Rizkallah, P.J., Berry, C. (2022) The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14: 863. DOI: 10.3390/toxins14120863

Hayes, A.J., Melrose, J. (2021) Neural tissue homeostasis and repair is regulated via CS and DS proteoglycan motifs. Frontiers Cell Dev Biol 9: 696640. DOI: 10.3389/fcell.2021.696640

Melrose, J., Hayes, A.J., Bix, G. (2021) The CNS/PNS extracellular matrix provides instructive guidance cues to neural cells and neuroregulatory proteins in neural development and repair. Internat J Mol Sci. 2021, 22: 5583. DOI: 10.3390/ijms22115583.

Farrugia, B., Hayes, A.J., Melrose, J. (2021) Chapter 4: Use of chondroitin sulphate to aid stem cell differentiation. In: “Proteoglycans in Stem Cells: From Development to Cancer” Biology of the Extracellular Matrix, volume 9. Eds: Götte, M. & Forsberg-Nilsson, K. Springer International Publishing. ISBN 978-3-030-73452-7.

Guilak, F., Hayes, A.J., Melrose, J. (2021) Perlecan in pericellular mechanosensory cell-matrix communication, extracellular matrix stabilisation and mechanoregulation of load-bearing connective tissues. Internat J Mol Sci. 22 (5): 2716. DOI: 10.3390/ijms22052716.

Hayes, A.J., Melrose, J. (2021) What are the potential roles of nuclear perlecan and other heparan sulphate proteoglycans in the normal and malignant phenotype. Internat J Mol Sci. 2021, 22(9): 4415. DOI: 10.3390/ijms22094415.

Saeedi, T., Prokopovich, P. (2021) Poly beta amino ester coated emulsions of NSAIDs for cartilage treatment. J. Mater. Chem. B 9: 5837–5847. DOI:10.1039/d1tb01024g. DOI: 10.1039/D1TB01024G.

Markov, P., Hayes, A.J., Zhu, H., Boote, C., Blain, E.J. (2021) 3D immuno-confocal image reconstruction of fibroblast cytoskleleton and nucleus architecture. J. Biophotonics. 2021;14:e202000202. DOI: 10.1002/jbio.202000202.

O’Morain, V.L., Chan, Y.-H., Williams, J.O., Alotibi, R., Alahmadi, A., Rodrigues, N.P., Plummer, S.F., Hughes, T.R., Michael, D.R., Ramji, D.P. (2021) The Lab4P Consortium of Probiotics Attenuates Atherosclerosis in LDL Receptor Deficient Mice Fed a High Fat Diet and Causes Plaque Stabilization by Inhibiting Inflammation and Several Pro-Atherogenic Processes. Molecular Nutrition & Food Research 65(17): 2100214. DOI: 10.1002/mnfr.202100214.

Ashworth, S., Harrington, J., Hammond, G.M., Bains, K.K., Koudouna, E. Hayes, A.J., , Ralphs, J.R., Regini, J.W., Young, R.D., Hayashi, R., Nishida, K., Hughes, C.E., Quantock, A.J. (2020) Chondroitin sulphate as a potential modulator of the stem cell niche in cornea. In: “Proteoglycans as Mediators of Cell Behaviour”. Frontiers in Cell and Developmental Biology. DOI: 10.3389/fcell.2020.567358.

Hayes, A.J., Melrose, J. (2020) Electro-stimulation, a promising therapeutic treatment modality for tissue repair: emerging roles of sulfates glycosaminoglycans as electro-regulatory mediators of intrinsic repair processes. Advanced Therapeutics. 202, 2000151. DOI: 10.1002/adtp.202000151.

Hayes, A.J., Melrose, J. (2020) Aggrecan, the primary weight-bearing cartilage proteoglycan, has context-dependent, cell-directive properties in embryonic development and neurogenesis: Aggrecan glycan side chain modifications convey interactive biodiversity. biomolecules. 10: 1244. DOI: 10.3390/biom10091244.

Hayes, A.J., Melrose, J. (2020) Keratan sulphate in the tumour environment. Adv Exp Med Biol. 1245: 39-66. DOI: 10.1007/978-3-030-40146-7_2.

Hayes, A.J., Melrose, J. (2020) Immunolocalization of keratan sulfate in rat spinal tissues using the keratanase generated BKS-1(+) neoepitope: Correlation of expression patterns with the class II SLRPS, lumican and keratocan. Cells. 9 (4): 826. DOI:10.3390/cells9040826.

Guschina, I.A., Hayes, A.J., Ormerod, S.J. (2020) Polystyrene microplastics decrease accumulation of essential fatty acids in common freshwater algae. Environmental pollution. 263 (part B): 114425. DOI: 10.1016/j.envpol.2020.114425.

Lloyd, D., Millet, C.O., Williams, C.F., Hayes, A.J., Pope, S.A.J., Pope, I., Borri, P., Langbein, W.F., Olsen, L., Isaacs, M.D., Lunding, A. (2020) Functional imaging of a model unicell: Spironucleus vortens as an anaerobic but aerotolerant flagellated protist. Adv Microb Physiol. 76: 1-39. DOI: 10.1016/bs.ampbs.2020.01.002.

Saeedi, T., Alotaibi, H.F., Prokopovich, P. (2020) Polymer colloids as drug delivery systems for the treatment of arthritis. Advances in Colloid and Interface Science 285: 102273. DOI: 10.1016/j.cis.2020.102273.

Hayes, A.J., Melrose, J. (2019) Glycosaminoglycan and proteoglycan biotherapeutics in articular cartilage protection and repair strategies: Novel approaches to visco-supplementation in orthobiologics. Advanced Therapeutics 2019, 2, 190034. DOI: 10.1002/adtp.201900034.

Groves, L.M., Williams, C.F., Hayes, A.J., Ward, B.D., Isaacs, M.D., Symonds, N.O., Lloyd, D., Horton, P.N., Coles, S.J., Pope, S.J.A. (2019) Fluorescent functionalised naphthaliimides and their Au(i)-NHC complexes for potential use in cellular Bioimaging. Dalton Trans 48: 1599-1612. DOI: 10.1039/C8DT04069A.

Hayes, A.J., Melrose, J. (2018) Glycans and glycosaminoglycans in neurobiology: key regulators of neuronal cell function and fate. Biochem J. 475: 2511-2545. DOI: 10.1042/BCJ20180283.

Hayes, A.J., Smith, S.M., Caterson, B., Melrose, J. (2018) Stem/progenitor cell proteoglycans decorated with 7-D-4, 4-C-3 and 3-B-3(-) chondroitin sulphate motifs are morphogenetic markers of tissue development. Stem Cells. 36 (10): 1475-1486. DOI: 10.1002/stem.2860.

Hallet, A.J., Placet, E., Prieux, R., McCafferty, D., Platts, J.S., Lloyd, D., Isaacs, M., Hayes, A.J., Coles, S.J., Pitak, M.B., Marchant, S., Marriott, S.N., Allemann, R.K., Dervisi, A., Fallis, I.A. (2018) Exploring the cellular uptake and localisation of phosphorescent rhenium fac-tricarbonyl metallosurfactants as a function of lipophilicity. Dalton Trans. 47: 14241. DOI: 10.1039/c8dt00669e.

Hayes, A.J., Sugahara, K., Farrugia, B., Whitelock, J. M., Caterson, B., Melrose, J. (2018) Biodiversity of CS-proteoglycan Sulphation Motifs: Chemical Messenger Recognition Modules with Roles in Information Transfer, Control of Cellular Behaviour and Tissue Morphogenesis. Biochem J. 475: 587-620. DOI: 10.1042/BCJ20170820.

Zaki, A.J., Hartley, A.M., Reddington, S.C., Thomas, S.K., Watson, P., Hayes, A., Moskalenko, A.V., Craciunc, M.F., Macdonald, J.E., Jones, D.D., Elliott, M. (2018) Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle. RSC Advances. 8: 5768-5775. DOI: 10.1039/C7RA11166E.

Kamanli, S.A., Kihara, T.C., Ball, A.D., Morritt, D., Clark, P.F. (2017) A 3D imaging and visualization workflow, using confocal microscopy and advanced image processing for brachyuran crab larvae. J Micros. 266: 307-323. DOI: 10.1111/jmi.12540.

Langdon-Jones, E.,m Williams, C., Hayes, A., Lloyd, D., Coles, S., Horton, P., Groves, L., Pope, S.J. (2017) Luminescent 1,8-naphthalimide-derived Re (I) complexes: syntheses, spectroscopy, X-ray structure and preliminary bioimaging in fission yeast cells. Eur J Inorganic Chemistry. 2017: 5279-5287. DOI: 10.1002/ejic.201700549.

Perry, I., Szeto, J-Y., Isaacs, M.D., Gealy, E.C., Rose, R., Scofield, S., Watson, P.D., Hayes, A.J. (2017) Production of 3D printed scale models from microscope volume datasets for use in STEM education. EMS Engineering Science Journal. 1 (1): 002.

Langdon-Jones, E.E., Jones, A.B., Williams, C.F., Hayes, A.J., Lloyd, D., Mottram, H.J., Pope, S.J.A. (2016) Anticancer, azonafide-inspired fluorescent ligands and their rhenium(I) complexes for cellular imaging. Eur J Inorg Chem. 2017: 759-766. DOI: 10.1002/ejic.201601271.

Sands, K., Wilson, M.J., Lewis, M.A.O, Wise, M.P., Palmer, N., Hayes, A.J., Barnes, R.A., Williams, D.W. (2016) Respiratory pathogen colonisation of dental plaque, the lower airways and endotracheal tube biofilms during mechanical ventilation. J Critical Care 37: 30-37. DOI: 10.1016/j.jcrc.2016.07.019.

Melrose, J., Smith, S.M., Hughes, C.E., Little, C.B., Caterson, B., Hayes, A.J. (2016) The 7D4, 4C3 and 3B3(-) chondroitin sulphation motifs are expressed at sites of cartilage and bone morphogenesis during foetal human knee joint development. J Glycobiol 5: 118. DOI: 10.4172/2168-958X.1000118.

Hayes, A.J., Shu, C.C., Lord, M.S., Little, C.B., Whitelock, J.M., Melrose, J. (2016) Pericellular localisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. Eur Cell Mater 32: 40-57. DOI: 10.22203/eCM.v032a03.

Hayes, A.J., Hughes, C.E., Smith, S.M., Caterson, B., Little, C.B., Melrose, J. (2016) The CS sulphation motifs 4C3, 7D4, 3B3[-]; and perlecan identify stem cell populations and niches, activated progenitor cells and transitional tissue development in the fetal human elbow. Stem Cells Dev. 25: 836-847. DOI: 10.1089/scd.2016.0054.

Kille, P., Morgan, A.J., Powell, K., Mosselmans, J.F., Hart, D., Gunning, P., Hayes, A., Scarborough, D., McDonald, I., Charnock, J.M. (2016) ‘Venus trapped, Mars transits’: Cu and Fe redox chemistry, cellular topography and in situ ligand binding in terrestrial isopod hepatopancreas. Open Biol 6: 150270. DOI:10.1098/rsob.150270.

Becket M.C., Ralphs, J.R., Caterson, B., Hayes, A.J. (2015) The transmembrane heparan sulphate proteoglycan syndecan-4 is involved in establishment of the lamellar structure of the annulus fibrosus of the intervertebral disc. Eur Cell Mater 30: 69-88. DOI: 10.22203/eCM.v030a06.

Stacey, O.J., Amoroso, A.J., Platts, J.A., Horton, P.N., Coles, S.J., Lloyd, D., Williams, C.F., Hayes, A.J., Dunsford, J.J., Pope, S.J. (2015) Water soluble, cyclometalated Pt(ii)-Ln(iii) conjugates towards novel bimodal imaging agents. Chem Commun (Camb) 51: 12305-12308. DOI: 10.1039/c5cc02623g.

Cavalcanti, Y.W., Morse, D.J., da Silva, W.J., Del-Bel-Cury, A.A., Wei, X., Wilson, M., Milward, P., Lewis, M., Bradshaw, D., Williams, D.W. (2015) Virulence and pathogenicity of //Candida albicans// is enhanced in biofiloms containing oral bacteria. Biofouling 31: 27-38. DOI:10.1080/08927014.2014.996143.

Langdon-Jones, E.E., Lloyd, D., Hayes, A.J., Wainwright, S.D., Mottram, H.J., Coles, S.J., Horton, P.N., Pope S.J. (2015) Alkynyl-naphthalimide fluorophores: gold coordination chemistry and cellular imaging applications. Inorg Chem 54: 6606-6615. DOI: 10.1021/acs.inorgchem.5b00954.

Frisbie, D.D., McCarthy, H.E., Archer, C.W., Barrett, M.F., Mcllwraith, C.W. (2015) Evaluation of articular cartilage progenitor cells for the repair of articular defects in an equine model. J Bone Surg Am 97: 484-493. DOI: 10.2106/JBJS.N.00404.

Lloyd, D., Lewis, I.B., Williams, C.F., Hayes, A.J., Symons, H., Hill, E.C. (2015) Motility of the diplomonad fish parasite Spironucleus vortens through thixotropic solid media. Microbiology 1610: 213-218. DOI 10.1099/mic.0.082529-0.

Hayes, A.J., Gibson, M.A., Shu, C., Melrose, J. (2014) Confocal microscopy demonstrates association of LTBP-2 in fibrillin-1 microfibrils and colocalisation with perlecan in the disc cell pericellular matrix. Tissue & Cell 46: 185-197. DOI: 10.1016/j.tice.2014.04.002.

Langdon-Jones, E.E., Symonds, N.O., Yates, S.E., Hayes, A.J., Lloyd, D., Williams, D., Williams, R., Coles, S.J., Horton, P.N., Pope, S.J. (2014) Fluorescent Rhenium-Naphthalimide conjugates as cellular imaging agents. Inorganic Chemistry 53: 3788-3797. DOI: 10.1021/ic500142z.

Sivan, S., Hayes, A.J., Wachtel, E., Caterson, B., Merkher, Y., Maroudas, A., Brown, S., Roberts, S. (2014) Biochemical composition and turnover of the extracellular matrix of the normal and degenerate intervertebral disc. European Spine Journal 23: 344-353. DOI 10.1007/s00586-013-2767-8.

Boros-Majewska, J., Salewska, N., Borowski, E., Milewski, S., Malic, S., Wei, X.Q., Hayes, A.J., Wilson, M.J., Williams, D.W. (2014) Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis. Medical Microbiology and Immunology 203: 341-355. DOI 10.1007/s00430-014-0343-4.

Young, R.D., Knupp, C., Pinali, C., Png, K.M.Y., Ralphs, J.R., Bushby, A.J., Starborg, T., Kadler, K.E., Quantock, A.J. (2014). Three-dimensional aspects of matrix assembly by cells in the developing cornea. Proceedings of the National Academy of Sciences 111: 687-692. DOI: 10.1073/pnas.1313561110.

Ho, L.T.Y., Harris, A.M., Tanioka, H., Yagi, N., Kinoshita, S., Caterson, B., Quantock, A.J., Young, R.D., Meek, K.M. (2014) A comparison of glycosaminoglycan distributions, keratan sulphate sulphation patterns and collagen fibril architecture from central to peripheral regions of the bovine cornea. Matrix Biol 38: 59-68. DOI: 10.1016/j.matbio.2014.06.004.

Hayes, A.J., Reynolds, S., Nowell, M.A., Meakin, L.B., Habicher, J., Ledin, J., Bashford, A., Caterson, B., Hammond, C.L. (2013) Spinal deformity in aged zebrafish is accompanied by degenerative changes to their vertebrae that resemble osteoarthritis. PLoS ONE 8 : e75787. DOI: 10.1371/journal.pone.0075787.

Shu, C., Hughes, C., Smith, S.M., Smith, M.M., Hayes, A.J., Caterson, B., Little, C.B., Melrose, J. (2013) The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc. Glycoconjugate Journal 30: 717-725. DOI 10.1007/s10719-013-9475-9.

Millet, C.O., Williams, C.F., Hayes, A.J., Hann, A.C., Cable, J., Lloyd, D. (2013) Mitochondria-derived organelles in the diplomonad fish parasite Spironucleus vortens. Experimental Parasitology 135: 262-273. DOI: 10.1016/j.exppara.2013.07.003.

Hayes, A.J., Mitchell, R.E., Bashford, A., Reynolds, S., Caterson, B., Hammond, C.L. (2013) Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis. Developmental Dynamics 242: 778-789. DOI: 10.1002/dvdy.23970.

Williams, C., Millet, C.O.M., Hayes, A.J., Cable, J., Lloyd, D. (2013) Diversity in mitochondrion-derived organelles of the parasitic diplomonads Spironucleus and Giardia. Trends in Parasitology 29 : 311-312. DOI: 10.1016/j.pt.2013.04.004.

Li, S., Hayes, A.J., Caterson, B., Hughes, C.E. (2013) The effect of beta-xylosides on the chondrogenic differentiation of mesenchymal stem cells. Histochemistry and Cell Biology 139: 59-74. DOI 10.1007/s00418-012-1017-1.

Hayes AJ, Smith SM, Melrose J (2013) Comparative immunolocalisation of fibrillin-1 and perlecan in the human foetal, and HS-deficient hspg2 exon 3 null mutant mouse intervertebral disc. Histochemistry & Cell Biology 139: 1-11. DOI 10.1007/s00418-012-1041-1.

Lloyd, D., Williams, C.F., Vijayalakshmi, K., Kombrabail, White, N., Hayes, A.J., Aon, M.A., Krishnamoorthy, G. (2013) Intracellular oxygen: similar results from two methods of measurement using phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7: 1350041 (14 pages). DOI: 10.1142/S1793545813500417.

Melrose, J., Isaacs, M.D., Smith, S.M., Hughes, C.E., Little, C.B., Caterson, B., Hayes, A.J. (2012) Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development. Histochem Cell Biol 138: 461-475. DOI 10.1007/s00418-012-0968-6.

Taylor, D.W., Ahmed, N., Hayes, A.J., Ferguson, P., Gross, A.E., Caterson, B., Kandel, R.A. (2012) Hyaline Cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes. J Histochem Cytochem 60: 576-597. DOI: 10.1369/0022155412449018.

Hooper, S., Percival, S., Hill, K., Thomas, D., Hayes, A.J., Williams, D. (2012) The visualisation and speed of kill of wound isolates on a silver alginate dressing. International Wound Journal 9: 633-642. DOI: 10.1111/j.1742-481X.2012.00927.x.

Lloyd, D., Coogan, M. P. and Pope, S. J. 2012. Novel metal-based luminophores for biological imaging. In: Geddes, C. D. ed. Reviews in Fluorescence 2010, Vol. 7. New York: Springer, pp. 15-44.

Hayes, A.J., Isaacs, M.D., Hughes, C., Caterson, B., Ralphs, J.R. (2011) Collagen fibrillogenesis in the development of the annulus fibrosus of the intervertebral disc. Eur Cell Mater 22: 226-241. DOI: 10.22203/eCM.v022a18.

Hayes, A.J., Lord, M.S., Smith, M.M., Whitelock, J.M., Weiss, A.S., Melrose, J. (2011) Colocalization in vivo and association in vitro of perlecan and elastin. Histochem Cell Biol 136: 537-454. DOI10.1007/s00418-011-0854-7.

Hayes, A.J., Ralphs, J.R. (2011) The response of foetal annulus fibrosus cells to growth factors: modulation of matrix synthesis by TGF-b1 and IGF-1. Histochem Cell Biol 136: 163-175. DOI 10.1007/s00418-011-0835-x.

Hayes A.J., Smith, S.M., Gibson, M.A., Melrose, J. (2011) Comparative immunolocalisation of the elastin fibre associated proteins fibrillin-1, LTBP2 and MAGP-1 with components of the collagenous and proteoglycan matrix of the foetal human intervertebral disc. Spine 36: E1365-1372. DOI: 10.1097/BRS.0b013e31821fd23e.

Parsons, P., Gilbert, S.J., Vaughan-Thomas, A., Sorrel, D.A., Notman, R., Bishop, M., Hayes, A.J., Mason, D.J., Duance, V.C. (2011) Type IX collagen interacts with fibronectin providing an important molecular bridge in articular cartilage. J Biol Chem 286: 34986-34997. DOI: 10.1074/jbc.M111.238188.

Shorning, B.Y., Grifffiths, D., Clarke, A.R. (2011) Lkb1 and Pten synergise to suppress mTOR-mediated tumorigenesis and epithelial-mesenchymal transition in the mouse bladder. PLoS One 6(1): e16209. DOI:10.1371/journal.pone.0016209.

Thorp-Greenwood, F.L., Fernandez-Moreira, V., Millet, C.O., Williams, C.F., Cable, J., Court, J.B., Hayes, A.J., Lloyd, D., Coogan, M.P. (2011) A ‘Sleeping Trojan Horse’ which transports metal ions into cells, localises in nucleoli, and has potential for bimodal fluorescence/PET imaging. Chem Commun 47: 3096-3098. DOI:10.1039/c1cc10141b.

Evans, L., Williams, A.S., Hayes, A.J., Jones, S.A., Nowell, M. (2011) Suppression of leukocyte infiltration and cartilage degradation by selective inhibition of pre–B cell colony-enhancing factor/visfatin/nicotinamide phosphoribosyltransferase: Apo866-mediated therapy in human fibroblasts and murine collagen-induced arthritis. Arthritis & Rheumatology 63: 1866-1877. DOI 10.1002/art.30338.

Hayes, A.J., Hughes, C., Ralphs, J., Caterson, B. (2011) Chondroitin sulphate sulphation motif expression in the ontogeny of the intervertebral disc. Eur Cell Mater 21: 1-14. DOI:10.22203/eCM.

Khan, I.M., Evans, S.L., Young, R.D., Blain , E.J., Quantock, A.J., Avery, A., Archer, C.W. (2011) Fibroblast growth factor 2 and transforming growth factor β1 induce precocious maturation of articular cartilage. Arthritis & Rheumatology. 63: 3417-3427. DOI 10.1002/art.30543.

Silva, S., Henriques, M., Hayes, A.J., Oliveira, R., Azeredo, J., Williams, D.W. (2010) Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium. J Oral Pathol Med 50: 421-427.

Akhtar, S., Bron, A.J., Hayes, A.J., Meek, K.M., Caterson (2010) Role of keratan sulphate (sulphated poly-N-acetyllactosamine repeats) in keratoconic cornea, histochemical, and ultrastructural analysis. Graefes Arch Clin Exp Ophthalmol 249: 413-420. DOI: 10.1007/s00417-010-1512-9.

Fernandez-Moreira, V., Thorp-Greenwood, F.J., Amoroso, A.J., Cable, J., Court, J.B., Gray, V., Hayes, A.J., Jenkins, R.L., Kariuki, B.M., Lloyd, D., Millet, C.O., Williams, C.F., Coogan, M.P. (2010) Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments. Org Biomol Chem 8:3888-901. DOI:10.1039/c004610h.

Coogan, M.P., Court, J.B., Gray, V.L., Hayes, A.J., Lloyd, S.H., Millet, C.O., Pope, S.J.A., Lloyd, D. (2010) Probing intracellular oxygen by quenched phosphorescence lifetimes of nanoparticles containing polyacrylamide-embedded [Ru(dpp(SO3Na)2)3]Cl2. Photochem Photobiol Sci 9: 103-109. DOI:10.1039/b9pp00071b.

Malic S.M., Hill, K.E., Hayes, A.J., Percival S.L., Thomas, D., Williams, D.W. (2009) Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid (PNA) fluorescent in situ hybridisation (FISH). Microbiol 155: 2603-2611. DOI: 10.1099/mic.0.028712-0.

Nowell, M.A., Williams, A.S., Carty, S.A., Scheller, J., Hayes, A.J., Jones, G.W., Richards, P.J., Slinn, S., Ernst, M., Jenkins, B.J., Topley, N., Rose-John, S., Jones, S.A. (2009) Therapeutic targeting of IL-6 trans signaling counteracts STAT3 control of experimental inflammatory arthritis. J Immunol 182: 613-22. DOI: 10.4049/jimmunol.182.1.613.

Kelly, C.M., Precious, S.V., Scherf, C., Penketh, R., Amso, N.N., Battersby, A., Allen, N.D., Dunnett, S.B., Rosser, A.E. (2009) Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression. Nat Methods 6: 271-273. DOI:10.1038/NMETH.1308.

Gealy, C.C., Hayes, A.J., Buckwell, R.E, Young, R.D, Caterson, B., Quantock, A.J., Ralphs, J.R. (2008) Actin and type I Collagen Propeptide Distribution in the Developing Chick Cornea. Invest Ophthalmol Vis Sci 50: DOI: 1653-1658. 10.1167/iovs.08-2554

Akhtar, S., Kerr, B.C., Hayes A.J., Hughes, C.E., Meek, K.M., Caterson, B. (2008) Immunochemical localisation of keratan sulphate proteoglycans in cornea, sclera and limbus using a keratanase generated neoepitope monoclonal antibody. Invest Ophthalmol Vis Sci 49: 2424-2431. DOI: 10.1167/iovs.06-1498.

Bazou, D., Coakley, W.T., Hayes, A.J., Jackson, S.K. (2008) Long-term viability and proliferation of alginate-encapsulated 3-D HepG2 aggregates formed in an ultrasound trap. Toxicol In Vitro 22: 1321-1331. DOI: 10.1016/j.tiv.2008.03.014.

Amoroso A.J., Arthur, R.J., Coogan, M.P., Court, J.B., Fernandez-Moreira, V., Hayes, A.J., Lloyd, D., Millet, C., Pope, S.J.A. (2008) 3-Chloromethylpyridyl bipyridine fac-tricarbonyl rhenium: a thiol-reactive luminophore for fluorescence microscopy accumulates in mitochondria. New J Chem 32: 1097-1102. DOI: 10.1039/B802215A.

Melrose, J., Hayes, A.J., Whitelock, J.M., Little, C.B. (2008) Perlecan, the “jack of all trades” proteoglycan of cartilaginous weight-bearing connective tissues. BioEssays 30: 457-469. DOI: 10.1002/bies.20748.

Hayes, A.J., Hughes, C., Caterson, B (2008) Antibodies and immunohistochemistry in extracellular matrix research. Methods 45: 10-21. DOI: 10.1016/j.matbio.2018.04.011.

Hayes, A.J., Tudor, D, Nowell, M.A., Caterson, B., Hughes, C.E. (2008) Chondroitin sulfate sulfation motifs as putative biomarkers for isolation of articular cartilage progenitor cells. J Histochem Cytochem 56: 125-138. DOI: 10.1369/jhc.7A7320.2007.

Hayes, A.J., Hall, A., Brown, L., Tubo, R, Caterson, B. (2007) Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. J Histochem Cytochem 55: 853-866. DOI: 10.1369/jhc.7A7210.20.

Gealy, E.C., Kerr, B.C., Young, R.D., Tudor, D., Hayes, A.J., Hughes, C.E., Caterson, B., Quantock, A.J., Ralphs, J.R. (2007) Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochem Cell Biol 128: 551-555. DOI 10.1007/s00418-007-0332-4.

Amoroso, A.J., Coogan, M.P., Dunne, J.E., Fernandez-Moreira, V., Hess, J.B., Hayes, A.J., Lloyd, D., Millet, C., Pope, S.J., Williams, C. (2007) Rhenium fac tricarbonyl bisimine complexes: biologically useful fluorochromes for cell imaging applications. Chem Commun (Camb) 2007: 3066-3068. DOI: 10.1039/B706657K.

Workman, V.L., Dunnett, S.B., Kille, P., Palmer, D.D. (2007) On-chip alginate microencapsulation of functional cells. Macromolecular Rapid Communications 28: 165-170. DOI: 10.1002/marc.200700641.

Workman, V. L., Dunnett, S. B., Kille, P, Palmer, D. D. (2007) Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells. Biomicrofluidics 1: 014105. DOI: 10.1063/1.2431860.

Blain, E.J., Gilbert, S.J., Hayes, A.J., Duance, V.C. (2006) Disassembly of the vimentin cytoskeleton disrupts articular cartilage chondrocyte homeostasis. Matrix Biol 25: 398-408. DOI: 10.1016/j.matbio.2006.06.002.

Aon, M.A., Cortassa S., Lemar K.M., Hayes, A.J., Lloyd D. (2006) Single and cell population respiratory oscillations in yeast: a 2 photon scanning laser microscopy study. FEBS Lett 581: 8-14. DOI: 10.1016/j.febslet.2006.11.068.

Malic, S., Hill, H.E., Ralphs, J.R., Hayes, A.J., Thomas D.W., Potts A.J., Williams D.W. (2006) Characterisation of Candida albicans infection of an in vitro oral epithelial model using confocal laser scanning microscopy. Oral Microbiol Immunol 22: 188-194. DOI: 10.1111/j.1399-302X.2007.00344.x.

Reed, K.R., Sansom, O.J., Hayes, A.J., Gescher, A.J., Peters, J.M., Clarke, A.R. (2006) PPAR Delta Status and mismatch repair mediated neoplasia in the mouse intestine. BMC cancer 6: 113. DOI: 10.1186/1471-2407-6-113.

Thomas, R.S., Liddell, J.E., Murphy, L.S., Pache, D.M., Kidd, E.J. (2006) An antibody to the beta-secretase cleavage site on amyloid-beta-protein precursor inhibits amyloid-beta production. J Alzheimers Dis 10: 379-390. DOI: 10.3233/JAD-2006-10406.

Young, R.D., Tudor, D., Hayes, A.J., Kerr, B., Hayashida, Y., Nishida, K., Meek, K.M., Caterson, B., Quantock, A.J. (2005) Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Invest Opthalmol Vis Sci 46: 1973-1978. DOI: 10.1167/iovs.04-1309.

Reed, K.R., Sansom, O.J, Hayes, A.J., Gescher, A.J., Winton, D.J., Peters, J.M., Clarke, A.R. (2004) PPAR delta status and Apc-mediated tumourigenesis in the mouse intestine. Oncogene 23: 8992-8996. DOI: 10.1038/sj.onc.1208143.

Sansom, O.J., Reed, K.R., Hayes, A.J., Ireland, H., Brinkmann, H., Newton, I.P., Batlle, E., Simon-Assmann, P., Clevers, H., Nathke, I.S., Clarke, A.R., Winton, D.J. (2004) Loss of Apc in vivo immediately perturbs Wnt signalling, differentiation, and migration. Genes & Development 18: 1385-1390. DOI: 10.1101/gad.287404.

Hayes, A.J., Dowthwaite, G.P., Webster, S.V., Archer, C.W. (2003) The distribution of Notch receptors and their ligands during articular cartilage development. J Anat 202: 495-502. DOI: 10.1046/j.1469-7580.2003.00185.x.

Hayes, A.J., MacPherson, S., Morrison, H., Dowthwaite, G., Ferguson, M.W.J., Archer, C.W. (2001)The development of articular cartilage: Evidence for an appositional growth mechanism. Anat Embryol 203: 469-479. DOI: 10.1007/s004290100178.

Hayes, A.J., Benjamin, M., Ralphs, J.R. (2001) Extracellular matrix in development of the intervertebral disc. Matrix Biol 20: 107-121. DOI: 10.1016/s0945-053x(01)00125-1.

Hayes, A.J., Benjamin, M., Ralphs, J.R. (1999) Role of actin stress fibres in the development of the intervertebral disc: Cytoskeletal control of extracellular matrix assembly. Dev Dyn 215: 179-189. DOI: 10.1002/(SICI)1097-0177(199907)215:3<179::AID-AJA1>3.0.CO;2-Q.

Lloyd D., Hayes A.J., Ralphs, J.R. (1999) Confocal laser scanning of environmental samples. In: Methods in Biotechnology. Volume 12: Environmental Monitoring of Bacteria (ed. Clive Edwards). Human Press Inc., Totowa, NJ.

Williams I., Paul F., Lloyd D., Jepras R., Critchley I., Newman M., Warrack J., Giokarini T., Hayes A.J., Randerson P.F., Venables W.A. (1999) Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface attached culture. Microbiol U.K. 145: 1325-1333. DOI: 10.1099/13500872-145-6-1325.

Shnyder S.D., Hayes A.J., Pringle J., Archer C.W. (1998) P-glycoprotein and metallothionein expression and resistance to chemotherapy in osteosarcoma. British Journal of Cancer 78: 757-759. DOI: 10.1038/bjc.1998.573.

Lloyd D., Thomas K.L., Hayes A.J., Hill B., Hales B.A., Edwards C., Saunders J.R., Ritchie D.A., Upton M. (1998) Micro-ecology of peat: Minimally invasive analysis using confocal laser scanning microscopy, membrane inlet mass spectrometry and PCR amplification of methanogen-specific gene sequences. FEMS Microbiol Ecol 25: 179-188. DOI: 10.1016/S0168-6496(97)00094-9.

Hayes A.J., Benjamin M., Ralphs, J.R. (1997) The involvement of actin in annulus formation in the rat lumbar intervertebral disc. J Anat 191:125.

Biagini, G.A., Hayes, A.J., Suller, M.T., Winters, C.W., Finlay, B.J., Lloyd, D. (1997) Hydrogenosomes of Metopus contortus physiologically resemble mitochondria. Microbiology 143: 1623-1629. DOI: 10.1099/00221287-143-5-1623.

Lloyd, D., Moran, A., Suller, M.T.E., Dinsdale, M.G., Hayes, A.J. (1996) Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation. J Inst Brew 102: 251-259. DOI: 10.1002/j.2050-0416.1996.tb00910.x.

Lloyd D., Williams A.G., Amann R., Hayes, A.J., Durrant L., Ralphs J.R. (1996) Intracellular prokaryotes in rumen ciliate protozoa: Detection by confocal laser scanning microscopy after in situ hybridisation with fluorescent 16s rRNA probes. Europ J Protistol 32: 523-531. DOI: 10.1016/S0932-4739(96)80011-3.

Lloyd, D., Hayes, A.J. (1995) Vigour, vitality and viability of micro-organisms. FEMS Microbiol Lett 133: 1-7. DOI: 10.1111/j.1574-6968.1995.tb07852.x.