What is confocal microscopy and how can it help me in my research?
Confocal laser scanning microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. By sequentially moving the plane of focus via a motorised specimen stage, images can be built up on a layer by layer basis, thereby enabling 3D reconstruction of the sample.