What is light sheet microscopy and how can it help me in my research?
Light sheet microscopy – also referred to as Single Plane Illumination Microscopy (SPIM) – is a fluorescent light microscopical imaging technique whereby the specimen is illuminated only in a single plane at a time and detected from the perpendicular direction. Images are built up on a layer by layer basis allowing reconstruction of three-dimensional structures from the obtained images, similar to confocal microscopy. Unlike confocal, the technique does not require a spatial pinhole to eliminate out of focus light.