What is spectral scanning and how can it help me in my research?
Spectral scanning is an advanced fluorescent imaging technique that records a series of individual images within a user-defined wavelength range with each image detected at a specific emission wavelength. The data collected can be used to measure the emission spectrum of new fluorochromes or to determine the emission maximum of a fluorescent dye in a specific sample to optimize detection. Spectral scanning can be extremely advantageous in the detection of auto fluorescence(s) where the spectrum can be unknown.
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Zimmerman et al. (2003) Spectral Imaging and its applications in live cell microscopy. FEBS Letters 546: 87-92