The illumination source, filter set and detection system of a microscope dictate whether its optical system can precisely excite a given fluorochrome combination and specifically detect/discriminate different fluorochrome emissions. It is therefore important that your choice of fluorochromes reflect the specific configuration of the microscope that you will use for their visualisation. This is especially important for labelling experiments involving multiple fluorochromes, particularly in fluorochrome co-localisation analyses, and for advanced fluorescence microscopy techniques. Helpful advice on fluorochrome selection can be found here.                                                                                                         

Wavelengths of visible light

Spectral Properties of Common Flurochromes

Fluorescence spectral viewer (via Molecular probes)

This tool is useful for the analysis of excitation and emission spectra of commonly used fluorochromes, particularly for multiplexed fluorescent labelling studies.