Confocal Microscopy

What is confocal microscopy and how can it help me in my research?

Confocal laser scanning microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. By sequentially moving the plane of focus via a motorised specimen stage, images can be built up on a layer by layer basis, thereby enabling 3D reconstruction of the sample.

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