{"id":374,"date":"2015-11-11T14:03:48","date_gmt":"2015-11-11T14:03:48","guid":{"rendered":"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/?p=374"},"modified":"2022-04-13T16:27:04","modified_gmt":"2022-04-13T16:27:04","slug":"in-focus-de-boning-a-fish-skeletal-development-of-the-zebrafish","status":"publish","type":"post","link":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/in-focus-de-boning-a-fish-skeletal-development-of-the-zebrafish\/","title":{"rendered":"IN-FOCUS: De-boning the Zebrafish: unpicking skeletogenesis under the microscope."},"content":{"rendered":"<div id=\"attachment_496\" style=\"width: 650px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-496\" class=\"wp-image-496 size-large\" src=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-1024x1024.jpg\" alt=\"Confocal reconstructions of the head, thorax and tail regions of the Zebrafish (Danio rerio)\" width=\"640\" height=\"640\" srcset=\"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-1024x1024.jpg 1024w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-150x150.jpg 150w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-300x300.jpg 300w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-70x70.jpg 70w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton-170x170.jpg 170w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Zebrafish-skeleton.jpg 1616w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><p id=\"caption-attachment-496\" class=\"wp-caption-text\">Confocal reconstructions of the head, thorax and tail regions of the Zebrafish (<em>Danio rerio<\/em>)<\/p><\/div>\n<p style=\"text-align: justify\">The Zebrafish (<em>Danio rerio<\/em>) is, in many ways, the perfect model for microscopists. Not only does it share 70% genetic homology with man, but its larvae are born in large, transparent broods all year round and\u00a0develop extremely\u00a0quickly (a single cell develops into something resembling a fish within 24 hours!) \u00a0This means that developmental events can be visualised <em>in vivo<\/em>\u00a0in real-time down the microscope. On top of this,\u00a0their\u00a0genome has been sequenced and it is\u00a0easily amenable to molecular\u00a0manipulation- again, these manipulations can be followed closely under\u00a0the microscope lens.<\/p>\n<p style=\"text-align: justify\">Over the last few years we have been collaborating with <a title=\"Dr Chrissy Hammond\" href=\"http:\/\/www.bris.ac.uk\/phys-pharm\/people\/chrissy-l-hammond\/index.html\">Dr Chrissy Hammond<\/a> at Bristol University, a fish biologist who shares an interest in skeletal development and disease. In our joint studies, we have used a variety of imaging\u00a0techniques (brightfield, <a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/dic\/\">DIC<\/a>, polarising, <a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/fluorescence\/\">epifluorescence<\/a>, <a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/confocal\/\">confocal<\/a>, TEM, radiography and microCT) to investigate skeletal development, growth and ageing in\u00a0\u00a0this animal model.<\/p>\n<p style=\"text-align: left\">One of the many interesting\u00a0findings from our studies is that ageing fish undergo degenerative changes to their spine that resemble osteoarthritis (for example, spinal curvature, osteophyte formation, and connective\u00a0tissue degeneration). This opens up the possibility \u00a0that they could be used to experimentally\u00a0model aspects of the human disease. So it&#8217;s not just fishy tails!<\/p>\n<p style=\"text-align: justify\">AJH<\/p>\n<h5 style=\"text-align: justify\">Find out more:<\/h5>\n<ul style=\"text-align: justify\">\n<li>Guardian article: <a title=\"How the diminutive zebrafish is having a big impact on medical research\" href=\"http:\/\/www.theguardian.com\/science\/2013\/sep\/15\/zebrafish-human-genes-project\">How the diminutive zebrafish is having a big impact on medical research<\/a><\/li>\n<li>Arthritis Research UK:\u00a0<a href=\"http:\/\/www.arthritisresearchuk.org\/arthritis-information\/arthritis-today-magazine\/154-autumn-2011\/fishy-tales.aspx\">Fishy tales<\/a><\/li>\n<\/ul>\n<h5 style=\"text-align: justify\">Further Reading:<\/h5>\n<ul>\n<li style=\"text-align: justify\">Hayes, et al. (2013) <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.23970\/epdf\">Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis<\/a>.\u00a0<em>Developmental Dynamics<\/em>\u00a0<strong>242<\/strong>: 778-789<\/li>\n<li style=\"text-align: justify\">Hayes et al. (2013) <a href=\"http:\/\/www.plosone.org\/article\/fetchObject.action?uri=info:doi\/10.1371\/journal.pone.0075787&amp;representation=PDF\">Spinal deformity in aged zebrafish is accompanied by degenerative changes to their vertebrae that resemble osteoarthritis<\/a>.\u00a0<em>PLoS ONE<\/em>\u00a0<strong>8\u00a0:<\/strong>\u00a0e75787<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The Zebrafish (Danio rerio) is, in many ways, the perfect model for microscopists. Not only does it share 70% genetic homology with man, but its larvae are born in large, transparent broods all year round and\u00a0develop extremely\u00a0quickly (a single cell develops into something resembling a fish within 24 hours!) \u00a0This means that developmental events can [&hellip;]<\/p>\n","protected":false},"author":908,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[15],"tags":[20],"class_list":["post-374","post","type-post","status-publish","format-standard","hentry","category-in-focus","tag-in-focus"],"meta_box":[],"_links":{"self":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/374","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/users\/908"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/comments?post=374"}],"version-history":[{"count":35,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/374\/revisions"}],"predecessor-version":[{"id":1431,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/374\/revisions\/1431"}],"wp:attachment":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/media?parent=374"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/categories?post=374"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/tags?post=374"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}