{"id":372,"date":"2015-11-11T14:03:48","date_gmt":"2015-11-11T14:03:48","guid":{"rendered":"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/?p=372"},"modified":"2022-04-13T16:27:04","modified_gmt":"2022-04-13T16:27:04","slug":"in-focus-gumming-up-the-works-oral-biofilms-under-the-microscope","status":"publish","type":"post","link":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/in-focus-gumming-up-the-works-oral-biofilms-under-the-microscope\/","title":{"rendered":"IN-FOCUS: Gumming up the works: oral biofilms under the microscope."},"content":{"rendered":"<figure class=\"image\">\n<div id=\"attachment_474\" style=\"width: 510px\" class=\"wp-caption aligncenter\"><a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/2015\/11\/11\/in-focus-gumming-up-the-works-oral-biofilms-under-the-microscope\/biofilm\/\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-474\" class=\"wp-image-474 size-full\" src=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm.jpg\" alt=\"Image showing yeast cells colonising a tissue-engineered oral epithelium\" width=\"500\" height=\"503\" srcset=\"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm.jpg 500w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm-150x150.jpg 150w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm-298x300.jpg 298w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm-70x70.jpg 70w, https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-content\/uploads\/sites\/492\/2015\/11\/Biofilm-170x170.jpg 170w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/a><p id=\"caption-attachment-474\" class=\"wp-caption-text\">Yeast cells (<em>Candida albicans<\/em>; red) colonise and invade a tissue-engineered oral epithelium (cell nuclei; blue; cytoplasm, green)<\/p><\/div>\n<\/figure>\n<p style=\"text-align: justify\">The BIOSI Bioimaging Facility\u00a0has worked closely with <a href=\"http:\/\/www.cardiff.ac.uk\/people\/view\/39506-williams-david\">Professor David Williams<\/a> at the Dental School in Cardiff for a number of years. David is an expert in oral microbiology, specialising in microbial biofilms (e.g. dental plaque) and mycoses such as <a href=\"http:\/\/www.nhs.uk\/Conditions\/Oral-thrush---adults\/Pages\/Introduction.aspx\">oral candidiases<\/a> (thrush). Over this time, we have been involved in a number of collaborative studies where we have used confocal microscopy and various fluorescent labelling techniques to investigate the formation, 3D organization and microbial community structure of biofilms grown on tissue engineered oral epithelium, endotracheal tubes and substrates such as dental acrylic and titanium. The research\u00a0has\u00a0also evaluated the effect of various anti-microbial and anti-fungal compounds and commercial mouth rinses on biofilm development using\u00a0<a href=\"https:\/\/www.thermofisher.com\/uk\/en\/home\/life-science\/cell-analysis\/cell-viability-and-regulation\/cell-viability\/microbial-viability.html\">fluorescent\u00a0viability stains<\/a>. The studies have extended our understanding of how oral biofilms develop\u00a0and in how they\u00a0respond to therapeutic intervention, and have resulted\u00a0in\u00a0a number of publications (see below) as well\u00a0as<a href=\"http:\/\/www.google.co.uk\/imgres?imgurl=http:\/\/t1.gstatic.com\/images%3Fq%3Dtbn:ANd9GcTh99VYRtwOPiQGPIKEBka3moRRJoCSzVRLKCbz0MYNyN5uRPdq&amp;imgrefurl=http:\/\/books.google.com\/books\/about\/Oral_Microbiology.html?id%3DEZeHhpjhDSgC%26source%3Dkp_cover&amp;h=785&amp;w=600&amp;tbnid=3VW2BF-KS-R-PM:&amp;tbnh=160&amp;tbnw=122&amp;usg=__eoI_kwmZaoemUHlhXpxvIsOH1Xw=&amp;docid=p-4CMwrLSJ3miM&amp;itg=1\">\u00a0<\/a><a title=\"Oral Microbiology (Amazon)\" href=\"http:\/\/www.amazon.co.uk\/Oral-Microbiology-Philip-Marsh-BSc\/dp\/0443101442\">a book cover<\/a>\u00a0for a leading text on the subject of Oral Microbiology. It&#8217;s a fantastic application of confocal microscopy to a biological problem and, from an imaging perspective, its been something for us to really get our\u00a0teeth into!<\/p>\n<p style=\"text-align: justify\">AJH<\/p>\n<h5 style=\"text-align: justify\">Find out more:<\/h5>\n<ul style=\"text-align: justify\">\n<li>Neu et al. (2014) <a href=\"http:\/\/download.springer.com\/static\/pdf\/666\/bok%253A978-3-319-09695-7.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Fbook%2F10.1007%2F978-3-319-09695-7&amp;token2=exp=1447929403~acl=%2Fstatic%2Fpdf%2F666%2Fbok%25253A978-3-319-09695-7.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Fbook%252F10.1007%252F978-3-319-09695-7*~hmac=7078d91e65d1b07b2a32cba82a7d9fadac17c2f9e2099628b5278ab81448d14c\">Investigation of microbial film structure by laser scanning microscopy<\/a>. <em>Productive Biofilms<\/em> <strong>146<\/strong>: 1-51<\/li>\n<li>Palmer &amp;Sternberg (1999) <a href=\"http:\/\/ac.els-cdn.com\/S0958166999800469\/1-s2.0-S0958166999800469-main.pdf?_tid=6c08e8e6-8ea5-11e5-8ca9-00000aacb35d&amp;acdnat=1447927863_2de1a999671c031922a4b6b82be86ab0\">Modern microscopy in biofilm research: confocal microscopy and other approaches.<\/a> <em>Curr Opin Biotechnol<\/em> <strong>10<\/strong>: 263-268<\/li>\n<\/ul>\n<h5 style=\"text-align: justify\">Further reading:<\/h5>\n<ul style=\"text-align: justify\">\n<li>Cavalcanti et al. (2015) <a href=\"http:\/\/www.tandfonline.com\/doi\/pdf\/10.1080\/08927014.2014.996143\">Virulence and pathogenicity of <em>Candida albicans<\/em> is enhanced in biofilms containing oral bacteria.<\/a> <em>Biofouling<\/em> <strong>31<\/strong>: 27-28<\/li>\n<li>Boros-Majewska et al. (2014) <a href=\"http:\/\/link.springer.com\/content\/pdf\/10.1007%2Fs00430-014-0343-4.pdf\">Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an <em>in vitro<\/em> model of oral candidosis<\/a>.\u00a0<em>Medical Microbiology and Immunology<\/em>\u00a0<strong>203<\/strong>: 341-355<\/li>\n<li>Hooper et al. (2012) <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1742-481X.2012.00927.x\/pdf\">The visualisation and speed of kill of wound isolates on a silver alginate dressing<\/a>.\u00a0<em>International Wound Journal<\/em>\u00a0<strong>9<\/strong>: 633-642<\/li>\n<li>Silva, et al. (2010)\u00a0<a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1600-0714.2010.00981.x\/epdf\"><em>Candida glabrata<\/em>\u00a0and\u00a0<em>Candida albicans<\/em>\u00a0co-infection of an in vitro oral epithelium<\/a> <em>J Oral Pathol Med\u00a0<\/em><strong>50<\/strong>: 421-427<\/li>\n<li>Malic et al. (2009) <a href=\"http:\/\/www.microbiologyresearch.org\/docserver\/fulltext\/micro\/155\/8\/2603.pdf?expires=1447336901&amp;id=id&amp;accname=guest&amp;checksum=1E50CD1E8EE794912E79368B29A61369\">Detection and identification of specific bacteria in wound biofilms using peptide nucleic acid (PNA) fluorescent\u00a0<em>in situ<\/em>\u00a0hybridisation (PNA FISH)\u00a0<\/a><em>Microbiol<\/em>\u00a0<strong>155<\/strong>: 2603-2611<\/li>\n<li>Malic et al (2006) <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-302X.2007.00344.x\/epdf\">Characterisation of <em>Candida albicans<\/em> infection of an\u00a0<em>in vitro<\/em>\u00a0oral epithelial model using confocal laser scanning microscopy<\/a>. <em>Oral Microbiol\u00a0 Immunol<\/em>\u00a0<strong>22:<\/strong>\u00a0188-194<\/li>\n<\/ul>\n<p style=\"text-align: justify\">\n","protected":false},"excerpt":{"rendered":"<p>The BIOSI Bioimaging Facility\u00a0has worked closely with Professor David Williams at the Dental School in Cardiff for a number of years. David is an expert in oral microbiology, specialising in microbial biofilms (e.g. dental plaque) and mycoses such as oral candidiases (thrush). Over this time, we have been involved in a number of collaborative studies [&hellip;]<\/p>\n","protected":false},"author":908,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[15],"tags":[12,20],"class_list":["post-372","post","type-post","status-publish","format-standard","hentry","category-in-focus","tag-confocal","tag-in-focus"],"meta_box":[],"_links":{"self":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/372","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/users\/908"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/comments?post=372"}],"version-history":[{"count":32,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/372\/revisions"}],"predecessor-version":[{"id":1082,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/372\/revisions\/1082"}],"wp:attachment":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/media?parent=372"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/categories?post=372"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/tags?post=372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}