{"id":355,"date":"2015-11-11T14:03:48","date_gmt":"2015-11-11T14:03:48","guid":{"rendered":"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/?p=355"},"modified":"2022-04-13T16:27:04","modified_gmt":"2022-04-13T16:27:04","slug":"in-focus-shedding-light-on-new-fluors-development-of-novel-metal-based-probes-for-bioimaging","status":"publish","type":"post","link":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/in-focus-shedding-light-on-new-fluors-development-of-novel-metal-based-probes-for-bioimaging\/","title":{"rendered":"IN-FOCUS: Shedding light on new fluors: development of novel, metal-based probes for bioimaging."},"content":{"rendered":"<p style=\"text-align: justify;line-height: 14.25pt\"><a href=\"http:\/\/www.cardiff.ac.uk\/people\/view\/38559-pope-simon\">Dr Simon Pope<\/a> and his research group in CHEMY have been collaborating with the BIOSI <a href=\"http:\/\/www.cardiff.ac.uk\/biosciences\/research\/facilities\/bioimaging-unit\">Bioimaging Unit<\/a> for over 8 years. Their research is focussed upon the development of new, metal-based, fluorescent probes for cell imaging applications, and forms part of a larger study on the use of metal complexes, which include rhenium and gold, as multi-modal imaging agents with therapeutic potential. In these studies, we have performed confocal imaging to (1) assess the cytotoxicity of the new probes, (2) evaluate their cellular uptake and determine their cytoplasmic localisation, and (3) characterise their fluorescent emissions via\u00a0<a href=\"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/spectral-wavelength-lambda-scanning\/\">spectral (wavelength or lambda) scanning<\/a>. The collaboration has yielded a number of high impact publications (see below) as well as a journal cover! With the improved potential of the new super-resolution confocal system we anticipate a lot more to come.<\/p>\n<p style=\"text-align: justify;line-height: 14.25pt\">AJH<\/p>\n<h5>Find out more:<\/h5>\n<ul>\n<li>Langdon-Jones et al. (2014) <a href=\"http:\/\/pubs.acs.org\/doi\/ipdf\/10.1021\/ic500142z\">Fluorescent Rhenium-Naphthalimide conjugates as cellular imaging agents.\u00a0<\/a><em>Inorganic Chemistry<\/em>\u00a0<strong>53<\/strong>: 3788-3797.<\/li>\n<li>Lloyd,\u00a0D., Coogan,\u00a0M.\u00a0P. and Pope,\u00a0S.\u00a0J. 2012.\u00a0<a href=\"http:\/\/www.springer.com\/cda\/content\/document\/cda_downloaddocument\/9781441998279-c1.pdf?SGWID=0-0-45-1265139-p174108184\">Novel metal-based luminophores for biological imaging.<\/a> In: Geddes,\u00a0C.\u00a0D. ed.\u00a0<i>Reviews in Fluorescence 2010<\/i>.\u00a0Reviews in Fluorescence,\u00a0Vol. 7.\u00a0New York: \u00a0Springer, pp. 15-44.<\/li>\n<li>Thorp-Greenwood et al. (2011) <a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2011\/cc\/c1cc10141b\">A \u2018Sleeping Trojan Horse\u2019 which transports metal ions into cells, localises in nucleoli, and has potential for bimodal fluorescence\/PET imaging.\u00a0<\/a><em>Chem Commun<\/em>\u00a0<strong>47<\/strong>: 3096-3098.<\/li>\n<li>Fernandez-Moreira et al. (2010) <a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2010\/ob\/c004610h\">Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments.<\/a>\u00a0<em>Org Biomol Chem<\/em>\u00a0<strong>8<\/strong>:3888-901.<\/li>\n<li>Coogan et al. (2010) <a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2010\/pp\/b9pp00071b\">Probing intracellular oxygen by quenched phosphorescence lifetimes of nanoparticles containing polyacrylamide-embedded [Ru(dpp(SO3Na)2)3]Cl2.\u00a0<\/a><em>Photochem Photobiol Sci<\/em>\u00a0<strong>9<\/strong>: 103-109.<\/li>\n<li>Amoroso et al. (2008) <a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2008\/nj\/b802215a\">3-Chloromethylpyridyl bipyridine\u00a0<em>fac<\/em>-tricarbonyl rhenium: a thiol-reactive luminophore for fluorescence microscopy accumulates in mitochondria.<\/a>\u00a0<em>New J Chem\u00a0<\/em><strong>32<\/strong>: 1097-1102.<\/li>\n<li>Amoroso et al (2007) <a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2007\/cc\/b706657k\">Rhenium fac tricarbonyl bisimine complexes: biologically useful fluorochromes for cell imaging applications.<\/a>\u00a0<em>Chem Commun (Camb)<\/em>\u00a02007<em>:<\/em>\u00a03066-3068.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Dr Simon Pope and his research group in CHEMY have been collaborating with the BIOSI Bioimaging Unit for over 8 years. Their research is focussed upon the development of new, metal-based, fluorescent probes for cell imaging applications, and forms part of a larger study on the use of metal complexes, which include rhenium and gold, [&hellip;]<\/p>\n","protected":false},"author":908,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[15],"tags":[20],"class_list":["post-355","post","type-post","status-publish","format-standard","hentry","category-in-focus","tag-in-focus"],"meta_box":[],"_links":{"self":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/users\/908"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/comments?post=355"}],"version-history":[{"count":28,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/355\/revisions"}],"predecessor-version":[{"id":1083,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/posts\/355\/revisions\/1083"}],"wp:attachment":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/media?parent=355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/categories?post=355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/tags?post=355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}