{"id":664,"date":"2015-11-19T13:00:06","date_gmt":"2015-11-19T13:00:06","guid":{"rendered":"http:\/\/blogs.cardiff.ac.uk\/bioimaging\/?page_id=664"},"modified":"2022-04-13T16:27:17","modified_gmt":"2022-04-13T16:27:17","slug":"spectral-wavelength-lambda-scanning","status":"publish","type":"page","link":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/spectral-wavelength-lambda-scanning\/","title":{"rendered":"Spectral (wavelength, lambda) scanning"},"content":{"rendered":"<h5 style=\"color: #383735\"><i>What is spectral scanning\u00a0and how can it help me in\u00a0my research?<\/i><\/h5>\n<p style=\"color: #383735;text-align: justify\">Spectral scanning is an advanced fluorescent imaging technique that\u00a0records a series of individual images within a user-defined wavelength range with each image detected at a specific emission wavelength. The data collected\u00a0can be used to measure the emission spectrum of new fluorochromes or to determine the emission maximum of a fluorescent dye in a specific sample to optimize detection. Spectral scanning\u00a0can be extremely advantageous in the detection of auto fluorescence(s) where the spectrum can be unknown.<\/p>\n<h5 style=\"color: #383735\"><em>Find out more:<\/em><\/h5>\n<ul style=\"color: #383735\">\n<li><a href=\"http:\/\/www.leica-microsystems.com\/science-lab\/spectral-detection-how-to-define-the-spectral-bands-that-collect-probe-specific-emission\/\">Leica Science Lab<\/a><\/li>\n<li><a href=\"https:\/\/www.microscopyu.com\/articles\/confocal\/spectralimaging.html\">Nikon Microscopy U<\/a><\/li>\n<li><a href=\"http:\/\/zeiss-campus.magnet.fsu.edu\/articles\/spectralimaging\/introduction.html\">Zeiss Online Campus<\/a><\/li>\n<\/ul>\n<h5 style=\"color: #383735\"><em>Our confocal systems:<\/em><\/h5>\n<ul style=\"color: #383735\">\n<li><a style=\"color: #045bc6\" href=\"http:\/\/sites.cardiff.ac.uk\/researchequipment\/equipment\/confocal-laser-scanning-microscope-4\/\">Zeiss LSM 880 confocal microscope<\/a><\/li>\n<li><a style=\"color: #045bc6\" href=\"http:\/\/sites.cardiff.ac.uk\/researchequipment\/equipment\/confocal-laser-scanning-microscope-2\/\">Leica SP2 confocal microscope<\/a><\/li>\n<\/ul>\n<h5><em>Further reading:<\/em><\/h5>\n<p>Zimmerman et al. (2003) <a href=\"http:\/\/ac.els-cdn.com\/S0014579303005210\/1-s2.0-S0014579303005210-main.pdf?_tid=d39e6f36-8ebd-11e5-ac65-00000aab0f01&amp;acdnat=1447938345_957314f2dc5c05d1c1205e12ae3cb2b8\">Spectral Imaging and its applications in live cell microscopy.<\/a> <em>FEBS Letters<\/em> <strong>546<\/strong>: 87-92<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is spectral scanning\u00a0and how can it help me in\u00a0my research? Spectral scanning is an advanced fluorescent imaging technique that\u00a0records a series of individual images within a user-defined wavelength range with each image detected at a specific emission wavelength. The data collected\u00a0can be used to measure the emission spectrum of new fluorochromes or to determine [&hellip;]<\/p>\n","protected":false},"author":908,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-664","page","type-page","status-publish","hentry"],"meta_box":[],"_links":{"self":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/pages\/664","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/users\/908"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/comments?post=664"}],"version-history":[{"count":6,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/pages\/664\/revisions"}],"predecessor-version":[{"id":1074,"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/pages\/664\/revisions\/1074"}],"wp:attachment":[{"href":"https:\/\/blogs.cardiff.ac.uk\/bioimaging\/wp-json\/wp\/v2\/media?parent=664"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}