Fluorescence Loss in Photo-bleaching (FLIP)

What is Fluorescence Loss in Photobleaching (FLIP) and how can it help me in my research?

Fluorescence Loss in Photobleaching (FLIP) is a technique employed to examine the movement of molecules inside cells . After incorporation of a fluorescent label (e.g., GFP) , a region of the cell is repeatedly illuminated by an intense laser beam (i.e., via laser scanning microscopy)  to induce photobleaching, while images are taken with reduced laser power with a time lag between the bleaches. The amount of fluorescence from that region is then measured over a period of time to provide information about protein dynamics in various cellular regions.

Find out more:
Our FLIP systems:
Further Reading:

Fluorescence Loss in Photobleaching.