What is Fluorescence Loss in Photobleaching (FLIP) and how can it help me in my research?
Fluorescence Loss in Photobleaching (FLIP) is a technique employed to examine the movement of molecules inside cells . After incorporation of a fluorescent label (e.g., GFP) , a region of the cell is repeatedly illuminated by an intense laser beam (i.e., via laser scanning microscopy) to induce photobleaching, while images are taken with reduced laser power with a time lag between the bleaches. The amount of fluorescence from that region is then measured over a period of time to provide information about protein dynamics in various cellular regions.